生化
2012
名词解释：7X3
- G protein cycle
- polylinker
- protome
- P/0
- citrate pyruvate cycle
- pentose phosphate shunt
- integrate regulation
论述题 6 选 4 10 分
- Please describe the formation, utilization and significance of the ketone bodies.
- Please describe how EGF functions through EGFR to activate MAPK pathway.
- the significance of glycolysis
- describe the metabolic speciality of liver
- the function of RE
- describe the Carcinogenesis gene
2011
名解
Oxidative phosphorylation
PRPP
Tumor suppressor gene
Cloning vector
Key enzyme
Reverse cholesterol transport
Substrate-level phosphorylation
问答题
- Please describe the formation, utilization and significance of the ketone bodies.
- PCR is of high specificity and sensitivity, why? What is the basic principle of real-time PCR?
- Please describe the essential stages ， key enzymes and energy - - production in aerobic oxidation of glucose, respectively
- Please try to describe the metabolic changes of main nutrients in the body when short-term starvation and prolonged starvation happened.
- When 15N-labeled is fed to animals the 15N label quickly appears in many amino acids.
Explain this observation. - Please describe how EGF functions through EGFR to activate MAPK pathway.
2010
名解
dideoxy termination method
covalent modification
apoptosis
de novo synthesis of purine nucleotide
substrate cycle
protein ADP-ribosylation
问答题
- Please try to describe the metabolic changes of main nutrients in the body when short-term starvation and prolonged starvation happened.
- Please present an example to explain how cAMP can promote glycogen breakdown based on the knowledge of signaling transduction pathway.
- What’s the significance of pentose-phosphate shunt?
- "Why has p53 been named the ""guardian of the genome""?"
- Please describe the essential steps in DNA cloning.
2010 补考
名解
checkpoint
G protein cycle
taq Polymerase
Lesch Nyhan syndrome
lactate cycle
union deamination
问答题
- description of the base steps of DNA cloning
- list the significance of pentose phosphate shunt
- list the important role of PRPP in nucleotide metabolism
- 某个技术（貌似是mass啥啥的）在Proteomics中的application
- description the coupling of eletron transport chain and oxidation phosphation
2009
名解
氧化磷酸化
抗代谢物
RT-PCR
VEGF
脂肪动员
磷酸戊糖
Checkpoint
论述
- 克隆载体的特点
- 酮体的生成代谢和意义
- 为什么要有从头合成和补救合成两种方式
- 为什么叫P53为细胞守卫
- 15N饲养动物会发现很多氨基酸中有15N，原因是？
2007
名解
DNA重组
cDNA文库
氧化磷酸化
SNP of genome
脂肪动员
底物循环
丙氨酸-葡萄糖循环
限速酶
问答题
- 真核生物怎么通过cAMP的浓度调控特定基因的表达
- 带有癌基因的逆转录病毒是如何通过插入病毒癌基因致癌
- 葡萄糖有氧氧化过程的步骤，关键酶，能量产生
- 什么是一碳单位，它如何联系氨基酸代谢跟核苷酸代谢
- 人类基因组计划中，public 基因测序与 privat 基因测序的相同与不同
2006
名解
Viral oncogen
Ristriction endonuclease
Catabolism
G protein
beta oxidation
Oxidative respiratory chain
Glutamate pyruvate transaminase
问答题
- State the reason why covalent modification casuses the function change of protein.Give an example in your answer sheet.
- State the mechanism of megaloblastic anaemia at the time of Vitamin B12 deficiency.
- Compare the difference and respective electron transfer chain between phosphate-3-glycerol shuttle and malate-asparate shuttle. State the mechanism of the difference between their ATP productions.
- List all the enzymes, participating in the glycogenesis and glycogenolysis in the liver, and their functions.
- State all the procedures of gene cloning at the time of using plasmid as vectors.
2005 补
名解
hnRNA
reverse transcription
S-D sequence
nucleic acid probes
proto-oncogen and oncogen
G protein
简答题
- describe the mechenism of transcription termination of prokaryotics
- what is cis-acting elements? Give 2-3 examples
- list three features of DNA replication
- 怎样从DNA文库中获取目的基因？
2005
名解
alternative splicing
gene expression
primer
shine-dalgarno sequence
nested-PCR
human genome
receptor
问答题
- 描述G蛋白偶联受体的特点，列举2条涉及它的信号通路。
- 描述原核生物翻译的起始、延伸和终止的过程。
- leading strand和lagging strand在DNA复制过程中有何差异？
- 什么是p53？它为什么被称为“gene guard”？
2004
名解
pcr-sscp,npn,
alternative splicing,
sd sequence,
chemical modification,
alpha complication,
okazaki fragment
简答题
- use examples to illustrate gene regulate at and after transcription initiation.
- activation of oncogene
- antibiotics:列举四个药说明作用机制
- liver metabolism
- 3 features of gene replication.